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Procedure of Gram stain for E coli Cover the smear with crystal violet and allow it to stand for one minute Rinse the smear gently under tap water Cover the smear with Gram's iodine and allow it to stand for one minute Rinse smear again gently under tap water Decolorize the smear with 95% alcohol Rinse the smear again gently under tap waterSuccessful Gram stains should be done on young, growing cells Many experiments in this course will utilize the Gram stain You should KNOW THE GRAM STAIN MATERIALS 1 Slant cultures of Escherichia coli, Micrococcus roseus, or other bacteria 2 Inoculating loops, Slides 3 Gram staining reagents in dropping bottles 4 Toothpicks 5Module The Gram Stain The "slide" contains E coli and Staph aureus – is that what you see? Escherichia Coli Bacteria Appearance Dangerous Infections And Diseases E Coli O157 H7 Treatment Organs And Body Parts Affected By E Coli O157 H7 Bacteria Cell Morphology And Gram Stain Gram Negative Bacteria E coli gram stain ...